Abstract: In order to obtain the monomer of polysaccharide from kadsura marmorata polysaccharides (KPS). KPS were fractionally precipitated by the different concentration ethanol. The polysaccharide monomers were separated through Sephadex column chromatography and NaHCO3 solution gradient elution. The content of sugar and absorbance value of eluant component were detected by anthrone-vitriol colorimetric method. The results of fractionally precipitation showed that five fractional polysaccharides, which named KPSⅠ~Ⅴ, and the content of sugar was 76.51 %, 78.36%, 95.13%, 92.41%, 88.35%, respectively. The results of separation by Sephadex column chromatography showed that KPSⅡ, KPSⅢ and KPSⅤ contained the single molecular weight polysaccharide, respectively. However, KPSⅠ and KPSⅣ contained the different molecular weight polysaccharides, respectively. Those polysaccharide monomers, which included KPSⅠ-1, KPSⅡ-1, KPSⅢ-1, KPSⅣ-6 and KPSⅤ-1 separated by Sephadex colum, laid the foundation for the further research chemical structure analysis of KPS.