Cloning and Sequence Analysis of HbSIP1 Promoter from Hevea brasiliensis

doi:10.11924/j.issn.1000-6850.2012-3111

Abstract

Abstract: The aim was to study the expression characteristics and regulation mechanism of pyrophosphatase gene in natural rubber biosynthesis. Based on the sequence of pyrophosphatase gene HbSIP1 from H. brasiliensis, the promoter sequence of HbSIP1 was cloned from H. brasiliensis by GenomeWalker method, the sequence was analyzed and a plant expression vector with the promoter sequence of HbSIP1 was constructed. Sequence analysis showed that the 1198 bp promoter sequence was obtained and its sequence had typical promoter features of eukaryote and contained several regulatory elements related to hormone and stress responses. Cloning and analysis of the promoter region of HbSIP1 could lay foundation for the further study of the function and expression regulation mechanisms of HbSIP1.

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