Effects of Dietary Chitosan on Growth, Lipid Deposition and Antioxidant Capacity of Juvenile Starry Flounder (Platichthys stellatus)

doi:10.11924/j.issn.1000-6850.2012-3141

Abstract

Abstract: The aim of this trial was to investigate the effects of replacement of dietary chitosan on growth performance, lipid deposition and antioxidant capacity of juvenile starry flounder Platichthys stellatus. A 64 days trial was conducted with juvenile starry flounder (46.03±0.11) g. Five isonitrogenous and isoenergetic diets were formulated by adding 0 (D0, control), 0.25% (D1), 0.50% (D2), 1.00% (D3) and 2.00% (D4) chitosan into the basal diet to replace equal amountα-fiber. The results showed that: Firstly, with the increasing dietary chitosan content, weight gain rate (WGR), specific growth rate (SGR) and daily feed intake (DFI) of trial animals increased, while Viscerosomatic index (VSI) obviously decreased (P＜0.05). No significant difference was found in feed conversion ratio (FCR) and survival ratio (SR) (P>0.05). Secondly, Lipid contents of whole fish (WL) in trial groups decreased with the increasing of dietary chitosan, however, significant difference was only found between D4 and D0 group (P＜0.05). There was significant positive correlation between WL and triglyceride (TG), cholesterol (CHO), low density lipoprotein cholesterol (LDL-C), respectively. Thirdly, Total antioxidant capacity of organisms in D4 group was significant higher than that in D0 group (P＜0.05), while no difference was found among the others(P>0.05). Anti-superoxide anion (O2-) activity of fishes in D2 group was significantly higher than that in other groups (P＜0.05). Activities of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and lysozyme (LZM) in fish serum in each treatment increased initially and then decreased, which peaked in D3, D3, D2, and D2 group, respectively. Serum albumin (Alb) and total protein (TP) concentration in trial fishes increased significantly with chitosan addition (P＜0.05). The results indicated that dietary chitosan could improve the growth performance and the antioxidant capacity, while decrease the lipid content in whole body of juvenile starry flounder. . According to WGR and antioxidant capacity, it could be concluded that the optimum level of chitosan in diet of starry flounder (46.03±0.11) g was 0.50%.

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