Abstract: For separation and utilization of cellulose and lignin in the bone of ramie, the cellulose and lignin in the bone of ramie were separated by formic acid-acetic acid system. The mass fraction of the ratio of formic acid-acetic acid-water, solid and liquid ratio, reaction temperature on separation effect were studied. Simultaneously, the FT-IR and some metals content were studied on the cellulose separated from the bone of ramie. The results showed that: the optimum conditions were formic acid /acetic acid/water (45/35/20), solid and liquid ratio of 1/20 (g/mL) and the extraction temperature of 100℃. The yield rate of cellulose was 66.54% and the extraction rate of lignin was 35.1% in the optimum conditions. The content of heavy metal Pb in the cellulose was 0. The results of this study were to provide the technical guidance for ramie resources comprehensive development and utilization, at the same to provide a theoretical reference for utilization of other plant resources.