Abstract: Defects in mitochondrial genes are the main sources of cytoplasmic male sterility in plants. Arabidopsis and rice have exactly the same gene sequence copies of atp6 and atp9 in nuclear genomes as compared to their mitochondrial genomes, but weather the nuclear copy is expressed has less been understood. On the other hand, Ecto-F1-ATPase is found on the surface of cell membrane, but the origin of its subunit genes is still unknown. This article cloned atp6 and atp9 gene sense and reverse fragments into pHANNIBAL, and then the expression frame was cloned into pCAMBIA 1300 to complete the construction of atp6 and atp9 RNAi vectors. The purpose is to study if defects of atp6 and atp9 can resulted in male sterility and to lay the technical foundation of genetic improvement in ramie