Abstract: Using seeds as explant, MS as basic culture medium, the techniques of tissue culture and rapid propagation of Callicarpa nudiflora were studied by comparing with types and concentrations of plant growth regulators, culture conditions, types of transplanting medium. The results showed that in the MS basic medium seed germination be made; the most suitable procedure for sterilization was to soak explants into 0.1% HgCl2 solution for 10 min after pretreatment with 75% alcohol for 10 s. Suitable temperature of buds subculture and proliferation was 28℃, the optimum light intensity was 1500 lx. MS+ 6-BA 2.00 mg/L+ NAA 0.05 mg/L be suitable for subculture and proliferation , the propagation coefficient was 10.87 every 30 days; the most optimum rooting medium was MS+ NAA 0.50-0.75 mg/L, and the rooting rate was 100%. The rooting seedling were effectively transplanted in river sand, perlite and topsoil (1:1:1), the highest survival rate was 96%, and the largest amount of growth. Using the technology of tissue culture and rapid propagation of Callicarpa nudiflora can realize the industrialized seeding.