Abstract: In order to explore the effect of Nano-carbon on crop growth, petri dish using test paper and agar culture were adopted and different concentration gradient of Nano-carbon were conducted to study effects of Nano-carbon on germination and root morphology of maize (Zea mays L. cv. ‘East agriculture 253’) seeds. The results showed that germination rate increased and had significant difference with control in 36-60h of maize seeds imbibition. The water absorption percentage, radicle and germ fresh weight, average radicle and germ length all increased gradually with the Nano-carbon concentration increasing in germination experiment. In 66mg/L treatments, all indexes reached maximum, which were higher 11.89%, 24.81%, 20.53%, 13.90%, 19.62%, 20.85% than control respectively, when the concentration was higher than 66mg/L, it were inhibited. In the maize root morphology experiment, the plant fresh weight, root fresh weight, primary seminal root length, LR number of primary seminal root were significantly increased with the Nano-carbon concentration increasing, which were higher than control by 8.39%, 9.24%, 24.14%, 10.59%. It suggested that Nano-carbon can promote seed germination and root growth in the certain concentration.