Abstract: This paper was aim to reveal the relationship between soil characteristics and the altitude in Zhangjiajie tobacco-growing area, and to provide theory evidence for tobacco planting programming and rational fertilization. 299 of soil samples were collected, and the elevation were recorded by using GPS technology. The difference of soil physical-chemical properties at different altitudes (below 400 m, between 400-600 m, between 601-800 m, above 800 m) was compared, and the status of soil fertility was evaluated by using descriptive analysis characteristics and spatial interpolation characteristics methods. The results showed that, basically, the content of soil nitrogen, organic matter, available sulfur, available Mo, available Mn were increased with the increasing of elevation, while the content of soil exchangeable Ca, available Fe, available Cu were decreased with the decreasing of elevation. The attitude below 400 m and above 800 m had obvious difference with other attitudes. By comparing the content of soil nutrient in different attitude, the soil in high altitude was preferably for high quality and characteristic tobacco production. In conclusion, 62% soil samples were suitable and the attitude 601-800 m had the highest frequency distribution in suitable soil of Zhangjiajie tobacco-growing area.