Abstract: Try to find the proper level of the nitrogen application for the high yield corn in Guizhou Province by studying the affection of the nitrogen absorption and the usage efficiency at the different levels of nitrogen application, using ‘Qianxing201’ as experimental material. The results showed that, the crop yield was increasing while increasing the quantity of nitrogen, it reached the highest when applied nitrogen at 450 kg/hm2. When applied nitrogen at 225-450 kg/hm2 the ear get the highest portion of nitrogen accumulate compare with leaf and stem, and physiological efficiency was high and stable. The nitrogen recovery efficiency and agronomic efficiency were decreasing while increasing the quantity of nitrogen. It was good combination on the yield and nitrogen efficiency when applied nitrogen between 225 kg/hm2 and 450 kg/hm2.