Abstract: Using UV spectrophotometer method and high performance liquid chromatography (HPLC) to analyze the biochemical component in fresh leaves of group varieties of Camellia sinensis var. assamica ‘Jianghua’. At the same time hierarchical cluster analysis showed that 100 individuals were divided into six categories. Class I performance of non-esterified catechins content was lowest. Class II polyphenols, phenolic ammonia ratio, the proportion of ester catechins and caffeine content were minimum. Class III EGC content was highest. Class IV polyphenols, phenolic ammonia ratio, theobromine were highest while theanine was at least. Class V caffeine, D,L-C, EC, ECG, catechins, non-ester type catechins content were highest. Class VI amino acid and theanine were highest and lowest total catechins. The obvious differences were existed in the six groups biochemical composition. It was helpful for the breeding of excellent tea tree resources.