Abstract: In order to investigate the effects of EM fertilizer on the maize (Zea mays L.) growth and yield, and furthermore to solve the using problems of the corn seed coated in double growing area effectively, the usage of EM fertilizer amount and water volume on four-leaf maize were studied in the south of Shanxi Province. The results showed that, spraying EM fertilizer during seedling stage on summer maize could enhance the growth and development, improved the agronomic traits, the yield components obviously and increased the yield significantly. Compared with spraying of 540 kg water, the 45 kg/hm2 EM fertilizer +540 kg/hm2 H2O treatment could increase significantly on the area of leaf (8.29%), the seeds per ear (15.14%) and highly significant on the ear length (8.57%), the yield by 18.5%. Compared with spraying of 1080 kg water, The 60 kg/hm2 EM fertilizer +1080 kg/hm2 H2O treatment could increase the high of plant, the stem diameter, the internode number, the height of ear, the area of leaf highly significant. The 45 kg/hm2 EM+1080 kg/hm2 H2O treatment could increase the length of ear, the ear diameter, seeds per ear, the weight per ear, the ear number of per hectare, the yield highly significant. The yield could increase 35.8%, which was the best.