Abstract: In order to solve the problems of non cultivated land area cultivated vegetable. Using mushroom residue, wheat straw, jerusalem artichoke straw, rape straw, river sand, cattle manure, sheep manure for experiment material, different substrate formulas to effects of tomato were studied in greenhouse. The results showed that the fruit quality and yield of tomato of substrate formulas A(cattle manure), C(mushroom residue: wheat straw: river sand=4:4:2) and L(cattle manure: sheep manure: river sand=4:4:2) were better than others substrate formulas, all their yield reached above 78.03 t/hm2 and were significantly greater than yield of others substrate formulas. Permeability and nutritional components of A(cattle manure), C(mushroom residue: wheat straw: river sand=4:4:2) and L(cattle manure: sheep manure: river sand=4:4:2) were suitable for nutrition absorption of tomato root and plant growth. Therefore, substrate formulas A(cattle manure), C(mushroom residue: wheat straw: river sand=4:4:2) and L(cattle manure: sheep manure: river sand=4:4:2) could be used as non cultivated land area tomato cultivation substrate formula in greenhouse in Qinghai.