Abstract: This article through to the Ganoderma lucidum field cultivation soil with coated plate method, extraction and separation of soil fungi were cultured in detection and identification of. The results showed that soil of Trichoderma, Penicillium, Aspergillus, Mucor, Neurospora, Rhizopus, Chaetomium and Gymnoascus subumbrinus in eight species of fungi. According to the fungal growth speed, Ganoderma lucidum in different growth period appeared the species and the colony number, put forward suggestions of prevention of soil fungi cultivation of Ganoderma lucidum. This experiment was to obtain beneficial fungi resources, high yield, high quality and efficient cultivation of Ganoderma lucidum, provide effective help.