Abstract: In order to select the best rate of slow-release fertilizer for the growth of Prunus zippeliana container seedlings, the influences of different rates of APEX on morphological, biological and physiological effects of 2-year Prunus zippeliana container seedlings were studied by adding different rates of APEX into the matrix. The height was in the order of SRF4>SRF2>SRF3>SRF1. There was no significant difference between basal diameter and it was in the order of SRF2>SRF4>SRF3. The shoot part fresh weight was in the order of SRF2>SRF4>CK>SRF3. The root part fresh weights were not significantly different which were between 6.56 g and 10.46 g. Shoot part dry weight was in the order of SRF2>SRF4>SRF3>CK while root part dry weight was SRF2>SRF4>SRF1>SRF3. The soluble sugar content of SRF2 and SRF1 were increased by 48.7% and 13.9% when compared with CK. The content of total chlorophyll was the lowest in SRF4 treatment and was in the order of SRF2>CK>SRF1>SRF3. There were significances between root activity of the treatments. The highest was in SRF2 treatment and was increased by 73.9% compared with CK treatment. The soluble protein content was in the order of CK>SRF2>SRF1>SRF3>SRF4. Overall morphological, biological and physiological index, the suitable amount was SRF2 that was APEX 6.0 kg/m3.