Abstract: In order to increase farmer’s income and reduce the waste of fertilizer, this field experiments were conducted to investigate the effects of nitrogen, phosphorus and potassium fertilizer on output and the accumulation of nitrogen, phosphorus, potassium in vegetables and soil residue under a tomatocauliflower-celery rotation system in greenhouse. The results showed that vegetable output without nitrogen, phosphorus and potassium, was decreased by 6.0%-13.8%, the outputs of tomato, cauliflower and celery were decreased by 39220 yuan/hm2, 36902 yuan/hm2, 22023 yuan/hm2, respectively. So, nitrogen, phosphorus and potassium were the main factors limiting the vegetable output. N accumulation in tomato and cauliflower＞celery; P accumulation in cauliflower＞tomato and celery; K accumulation in tomato＞celery＞cauliflower. N accumulation in the fruits and plant of tomato was lower than cauliflower, moreover, P, K accumulation was inverse. The order of total accumulation of N, P and K were cauliflower＞tomato＞celery. After harvest, the residual N, P and K in soil were higher than it was before sowing. The N content contained in the soil which was planted tomato and the P content contained in the soil which was planted celery were doubled before they sowed, that was because the manure was applied. Before cauliflower sowed, manure wasn’t applied, N and K content in the soil were lower than the soil of tomato and celery were harvested. Soil environment was polluted and resource was wasted due to the high level of phosphorus residue.