Abstract: In order to investigate the method of making Aspiorhynchus laticeps paraffin section,understanding its basic structure and summarizing the problems in the production of paraffin section, analyzed the reasons and solutions. It was a good way for using the technique of histological paraffin sectioning and Hematoxylin-eosin staining to observe the organizational structure of Aspiorhynchus laticeps. The results showed that there were two rows of gill lamellae, each side with a lot of small gill lamella, each gill filaments have small gill lamellae, afferent branchial artery and efferent branchial artery were visible after staining, the muscle slice was more clearly than gills slice of Aspiorhynchus laticeps. The presence of capillary was clear between the muscle fibrils and connective tissue of Aspiorhynchus laticeps. The nucleoli of liver cells was larger than the other nucleoli, all of the cell was closely packed. From the experiment we could got a preliminary understanding about the gill, muscle and liver of Aspiorhynchus laticeps, it was provided a reliable histological basis data for the research of Aspiorhynchus laticeps.