Genetic Diversity of Six Populations of Siniperca Chuatsi: Analysis Based on SSR

doi:10.11924/j.issn.1000-6850.casb20191000741

Abstract

Abstract:

To study the genetic diversity and the status of germplasm resources in different populations of Siniperca chuatsi, based on microsatellite marking technology, preliminary analysis of genetic diversity was carried out in 96 individuals from 6 populations of Siniperca Chuatsi. The results showed that the average value of allele (Na) was from 2.2000 to 2.5000 by ten pairs selected microsatellite markers. The average expected heterozygosity (He) value was from 0.6489 to 0.7289, and the average polymorphic information content (PIC) was from 0.5614 to 0.6409 (PIC大鱼0.5). The genetic differentiation coefficient (Fst) among the populations was 0.0606 (0.05小鱼Fst小鱼0.15), and the gene flow (Nm) was 4.0151. The test indicated that other four populations deviated from Hardy-Weinberg Equilibrium in some extent except Gaoyou and Jingjiang population, which mainly showed heterozygote deficiency. The UPGMA clustering showed that dangtu population formed an independent cluster, and the other five populations were another cluster. The study indicates that the genetic diversity of the six populations of Siniperca chuatsi is relatively high, there are a medium genetic differentiation and some gene flow among the six populations.

Key words: Siniperca chuatsi, different populations, microsatellite marker, genetic diversity

CLC Number:

S917
Q346

Zhou Mengchen, Xu Dongpo, Fang Di’an, Zhou Yanfeng, Liu Kai, Zhang Minying. Genetic Diversity of Six Populations of Siniperca Chuatsi: Analysis Based on SSR[J]. Chinese Agricultural Science Bulletin, 2020, 36(26): 147-152.

Figures/Tables 7

References 25

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位点	正向引物(5′-3′)	反向引物(5′-3′)	退火温度/℃	GenBank序列号
G01	AGCCATCACCATCTGCCTA	TGTGCGGTGTCTGTATTCG	56	DQ789260
G03	GCCATCTATCATCAACAAAATC	TCCCAGTTATTCACTCCCAT	54	DQ789261
G04	TTCCCATGCAGCAAAGGT	TAGGGGGGCTGAGAGCATAA	54	DQ789257
G06	AGCCATCACCATCTGCCTAC	CACTAAAACCCTGCCTCCC	57	DQ789262
G08	GAACGAGTATCGCATTAGCC	GAAAGTTGTGCAATGCGC	57	DQ789254
G09	TGGTCTGGTCCTGTTTGTGTC	GGCATGTAGCATGACATGCA	52	DQ789255
G10	GCAGTTTCCTGGTTACTCCTC	GCTTTGCTGAGTGGTTAGAGT	55	DQ789256
G12	AGGAAGGATTTAGTCAAGGTG	ACAAGCTCCCAGCCTGAT	58	DQ789258
G13	CGTGAGCTTTCTTTCAGTCTG	CGTTCTTGGTGGTTCCTATG	56	DQ789259
G14	ACACAGCGAACATGATCCAGTC	GGGCTTCCCAGATCTGCTC	53	DQ789264

位点	正向引物(5′-3′)	反向引物(5′-3′)	退火温度/℃	GenBank序列号
G01	AGCCATCACCATCTGCCTA	TGTGCGGTGTCTGTATTCG	56	DQ789260
G03	GCCATCTATCATCAACAAAATC	TCCCAGTTATTCACTCCCAT	54	DQ789261
G04	TTCCCATGCAGCAAAGGT	TAGGGGGGCTGAGAGCATAA	54	DQ789257
G06	AGCCATCACCATCTGCCTAC	CACTAAAACCCTGCCTCCC	57	DQ789262
G08	GAACGAGTATCGCATTAGCC	GAAAGTTGTGCAATGCGC	57	DQ789254
G09	TGGTCTGGTCCTGTTTGTGTC	GGCATGTAGCATGACATGCA	52	DQ789255
G10	GCAGTTTCCTGGTTACTCCTC	GCTTTGCTGAGTGGTTAGAGT	55	DQ789256
G12	AGGAAGGATTTAGTCAAGGTG	ACAAGCTCCCAGCCTGAT	58	DQ789258
G13	CGTGAGCTTTCTTTCAGTCTG	CGTTCTTGGTGGTTCCTATG	56	DQ789259
G14	ACACAGCGAACATGATCCAGTC	GGGCTTCCCAGATCTGCTC	53	DQ789264

位点	等位基因数	有效等位基因数	观测杂合度	期望杂合度	多态信息含量	遗传分化指数	基因流
G01	2.0000	3.3149	0.6333	0.7042	0.3639	0.0632	3.7028
G03	4.0000	3.4450	0.6167	0.7157	0.4355	0.0466	5.1176
G04	3.0000	3.3058	0.6167	0.7034	0.4930	0.0633	3.6981
G06	3.0000	3.3566	0.6500	0.7080	0.4116	0.0457	5.2208
G08	3.0000	3.1816	0.6333	0.6915	0.3216	0.0703	3.3069
G09	3.0000	3.2447	0.6167	0.6976	0.5082	0.0652	3.5815
G10	2.0000	3.3661	0.5667	0.7088	0.3748	0.0587	4.0101
G12	3.0000	3.3012	0.5500	0.7029	0.5238	0.0496	4.7892
G13	4.0000	3.1332	0.7000	0.6866	0.4417	0.0588	4.0052
G14	3.0000	3.1048	0.6500	0.6836	0.4431	0.0842	2.7190
平均值	3.0000	3.2754	0.6233	0.7002	0.4317	0.0606	4.0151

位点	等位基因数	有效等位基因数	观测杂合度	期望杂合度	多态信息含量	遗传分化指数	基因流
G01	2.0000	3.3149	0.6333	0.7042	0.3639	0.0632	3.7028
G03	4.0000	3.4450	0.6167	0.7157	0.4355	0.0466	5.1176
G04	3.0000	3.3058	0.6167	0.7034	0.4930	0.0633	3.6981
G06	3.0000	3.3566	0.6500	0.7080	0.4116	0.0457	5.2208
G08	3.0000	3.1816	0.6333	0.6915	0.3216	0.0703	3.3069
G09	3.0000	3.2447	0.6167	0.6976	0.5082	0.0652	3.5815
G10	2.0000	3.3661	0.5667	0.7088	0.3748	0.0587	4.0101
G12	3.0000	3.3012	0.5500	0.7029	0.5238	0.0496	4.7892
G13	4.0000	3.1332	0.7000	0.6866	0.4417	0.0588	4.0052
G14	3.0000	3.1048	0.6500	0.6836	0.4431	0.0842	2.7190
平均值	3.0000	3.2754	0.6233	0.7002	0.4317	0.0606	4.0151

群体	平均等位基因数	有效等位基因数	观测杂合度	期望杂合度	多态信息含量
TL	2.2000	3.2602	0.6200	0.7289	0.6409
DT	2.5000	2.8199	0.5300	0.6758	0.6326
AQ	2.3000	2.7352	0.6000	0.6542	0.6083
TH	2.2000	2.6577	0.6400	0.6489	0.5614
GY	2.5000	3.0921	0.7100	0.7116	0.6144
JJ	2.4000	3.0104	0.6400	0.7011	0.6247