Abstract: In order to explore the feasibility of propagating Pinus densiflora var. zhangwuensis by tissue culture, the dormant shoot buds of the species were used as explants to investigate the influence of sterilization methods, medium composition and plant growth regulators on axillary bud induction. The results showed that: the most effective sterilization treatment was rinsing the explants in 70% ethanol for 30 s following with 0.1% HgCl2 for 5 min. The optimal medium for axillary bud induction was WPM containing 2-5 mg/L BA and 30 g/L sucrose (shoot induction 50%-70%). NAA didn’t promote axillary bud induction. The new axillary shoots alternately subcultured in WPM with and without PGR elongated and proliferated well.