Chinese Agricultural Science Bulletin ›› 2013, Vol. 29 ›› Issue (22): 147-151.doi: 10.11924/j.issn.1000-6850.2013-0020
Special Issue: 园艺
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Abstract: Various macroporous resins with different properties were employed to purify the resveratrol from the roots of Polygonum Cuspidatum. Static and dynamic adsorption and desorption experiments were conducted, meanwhile, the adsorption and desorption rates of resveratrol were concerned to test out the prominent macroporous resin and optimal processing parameters. H103 was subsequently selected as the best macroporous resin. The optimal processing parameters were obtained as follows: the crude extract containing resveratrol of 0.72 mg/mL was loaded at a flow rate of 2BV/h, the H103 column was washed with 4BV of distilled water for removing the impurities, elution with 8BV of 75% ethanol was at a flow rate of 1.5BV/h. As a result, the content of purified resveratrol was increased from 12.8% to 53.5%. The method with H103 as the optimal macroporous resin undergoing with the modified purification process is a simple, safe and inexpensive technology, and has a promising potential of purifying resveratrol from the roots of Polygonum Cuspidatum.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.2013-0020
https://www.casb.org.cn/EN/Y2013/V29/I22/147