Abstract: In order to achieve fertilization scheme of Chinese chives seedling with high quality, the effect of nitrogen, phosphorus and potassium on seedling was studied with design of saturated D-optimal regression. And the resulted obtained, a mathematical model was established, in which N and K applied were independent variables, integrated quality value of seedlings was dependent variables. The results showed that the seedling quality was significantly improved with reasonable usage and ratio of N, P and K, Meanwhile the effect of potassium was higher than phosphorus and nitrogen, the interaction of potassium and phosphorus was significant. When N was 205.65-304.95 kg/hm2, P2O5 was 164.10-225.00 kg/hm2, K2O was 228.45-328.50 kg/hm2 under this experimental condition, the fertilizer project with integrated quality value of seedlings over 90 was achieved.