Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2013, Vol. 29 ›› Issue (26): 9-13.doi: 10.11924/j.issn.1000-6850.2013-0185

Special Issue: 生物技术 畜牧兽医

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The Expression of Maternal Imprinting Gene in the Bovine Early Stage Embryos

  

  • Received:2013-01-18 Revised:2013-03-14 Online:2013-09-15 Published:2013-09-15

Abstract: In order to detect the expression profiles of the maternal imprinted genes Magel2, Sgce and SNRPN in the earlier bovine embryos, as well as preliminary determine the stage from which the imprinting start. In this study, the expression profiles of three maternal imprinted genes were detected between bovine Parthenogenetic Activation (PA) and in vitro Fertilization (IVF) embryos by Real-time PCR. The results showed that the expression patterns of the three genes were significantly different between IVF and PA embryos. Magel2 was expressed from 2 to blastocyst in bovine IVF embryos, while no detectable transcripts were observed in PA embryos. Sgce was expressed from 2 to blastocyst in bovine IVF embryos, while 2 to 4 cell stage in PA embryos. In bovine IVF embryos SNRPN transcripts could be detected from 2 to blastocyst stage, but it was only detected in 2-4 cell stage PA embryo.SNRPN expression could be detected in bovine IVF embryos from 2 cell to blastocyst stages, meanwhile it was only detected in PA 2 cell and blastocyst stage embryos. The results showed that maternal imprint genes Magel2, Sgce and SNRPN may might exert important roles on bovine early embryonic development.