Abstract: The reversion of vitrification of Asparagus officinalis L. during tissue culture was discussed. The influence factors on the reversion of vitrification were studied, including culture temperatures, culture illumination, concentration of sucrose and agar. The results showed that the percentage of the reversion of vitrification would be increased with decreasing temperature, the rate of reversion was 33.01%, when the culture temperature at 15℃. The rate of reversion would be increased obviously along with the increase of culture illumination, the rate would amount to 70.55%, under the intensity of 5200 lx culture illumination. With the increase of sucrose concentration, the recovery rate increased gradually, when the sucrose concentration was 50 g/L, the recovery rate increased to 48.20%. The rate of reversion increased with the agar concentration increasing, when the agar concentration was 9 g/L, the recovery rate was 38.18%. The culture illumination showed the best effect on the reversion of vitrication in the 4 factors. Moreover, the culture temperatures, the concentration of sucrose and agar showed some efficacious effects on the reversion of vitrication.