Abstract: In order to get a strain of degrading PVA bacillus, and immobilization of the strain resources. Using PVA iodine-boric acid plate method for screening, measures degradation for rescreening, and explored the immobilization process by orthogonal experiment. Eight bacterial strains showed degrade activity to polyvinyl alcohol, and 3-9 was obtained during the rescreening. After 48 hours, PVA degradation rate up to 86.56%. This strain was identified as Bacillus subtilis subsp.. The optimum immobilization conditions for this strain are as follows: sodium alginate of 4%, CaC1₂ of 3%, cells of 0.1 g and calcification time was 4 hours. The results showed that degradation rate increased by 14.02% after immobilized.This research was supported by an efficient degradation of polyvinyl alcohol Bacillus subtilis subsp., and determined the optimal immobilization conditions，provided resources and theoretical basis for PVA wastewater treatment.