Abstract: In order to expand the breeding flock of cattle in China, multiple ovulation and embryo transfer breeding programs were applied to the limited number of imported cattle. This study investigated the effects of superovulation from five different breeds, parity (nulliparous and multiparous), different nutrition levels, the interval time of repeated superovulation, the time of treatment on cattle superovulation. The results showed that four different breeds did not significantly affect the results of superovulation (P>0.05). Nulliparous for superovulation treatment were better multiparous (P<0.01). The group of different nutrition levels extremely affected the results of superovulation. The trial investigated the optimal interval time of repeated superovulation was 39 days. The superovulation treatment consisted of eight intramuscular injections with the first FSH treatment on day 9 or 10. There were significantly affection of parity (nulliparous and multiparous), different nutrition levels, the time of treatment superovulation on cattle superovulation, and the effects were not significant of different breeds, the interval time of repeated superovulation on cattle superovulation.