Abstract: 【Objective】Selected the most suitable medium for Cerasus humilis proliferation could improve the quality of the plantlets and optimize asexual reproduction system of Cerasus humilis. 【Method】The proliferation culture stage plantlets of Chinese Dwarf Cherry 2 were as test materials，and different media, different hormone concentrations and different sucrose concentrations and different pH were as the main content to study the proliferation effect. 【Result】The most appropriate medium for Cerasus humilis 2 to Proliferation was MS . In the treatment of NAA 0.2 mg / L +6- BA 0.5 mg / L + MS , the average differentiated shoots number and the number of shoots greater than 2 cm were the largest. The treatment of MS medium with sucrose concentration of 40 g / L can not only significantly increase the number of differentiated shoots but also improve seedling rate. The MS medium with pH 6.0 was most suitable for the growth of Cerasus humilis 2. 【Conclusion】 Suitable for Prunus Humilis Bunge 2 proliferation medium was MS +NAA 0.2 mg/L+6-BA 0.5 mg/L+6 g agar +40 g sucrose, pH value is 6.0.