Abstract: A stable suspending cell culture of Fraxinusm andshu rica could be used for genetic transformation or screening monoclone after mutagenesis. In this study, stalks of Fraxinusm andshu rica were used to induce calli and suspending cells were obtained after a series gradient of 2,4-D were added in the culture. The calli could be subcultured 3-4 gengerations every 15d in the media of WPM+BA0.1mg/L+2,4,-D0.015mg/L+20g/L sucrose to get stalbe cell culture.