Study on the Transferability and Application of ACGM Molecular Makers from Rice in Foxtail Millet

doi:10.11924/j.issn.1000-6850.2013-0765

Abstract

Abstract: The purpose of the present research was to study the transferability and the polymorphism of rice (Oryza sativa) genome data in foxtail millet (Setaria italica). 38 ACGM markers exploited from rice were investigated in 59 foxtail millet accession using PCR methods. The transferability and the polymorphism of rice (Oryza sativa) genome data in foxtail millet were analyzed and the polymorphism data of these markers among the accession were used for clustering through biostatistics DARwin 5.0.156. The results showed that a total of 33 pairs of primers behaved specific PCR products in at least five accessions, about occupying 87% of total primers. Among those, 7 pairs of primers occupying 18.4% of the total markers were of polymorphism among the accessions. The molecular clustering results of the tested materials showed that it was not fully consistent with that depending on the phenotypic or ecological data and the reason need further study. The results showed that the makers from rice had a high transferability in foxtail millet and the polymorphism of these markers was lower in foxtail millet compared with other crops.

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