Abstract: In order to find out the dominant weather factors of production and quality of Rosa roxburghii Tratt, divide the climate suitability region of Rosa roxburghii Tratt in Guizhou. The author took 20 growing places of Rosa roxburghii Tratt in Guizhou as samples, then investigated their distribution conditions, observed their phenophase, analyzed the degree of drought disaster and tested their qualities. According to the agro-climatic similarity theory, through the analysis of climate conditions on the quality of Rosa roxburghii Tratt, the results showed that: average temperature from May to August, average temperature in July, total precipitation from March to August, and the accumulated temperature≥10℃ in growth period had very important influence on Rosa roxburghii Tratt’s quality. The weight and diameter of Rosa roxburghii Tratt increased with the increase of total precipitation from March to August, precipitation too much or too low were detrimental to the synthesis of VC and SOD，average temperature from May to August and the accumulated temperature≥10℃ too high or too low were detrimental to the synthesis of water soluble total sugar and reducing sugar. According to suitable climate data of Rosa roxburghii Tratt, then the author drafted zoning map of eco-climatic suitability by 80% guarantee rate statistics. Divisions as follows: suitable areas were central Guizhou Province, most of Bijie District, southwest parts of Guizhou, north of Zunyi City; non-suitalbe areas were marginal areas of the northwest and southwest of Guizhou Province, part of southeast in Guizhou.