Abstract: To increase the fruit setting rate and improve the quality of apple fruit, the different apple production area apple trees were studied by using the field experiment. The results showed that Osmia bees began to fly in a low temperature (10-11℃) and had long working hours a day (10h). The mount of visiting flowers was high (4200) and pollination ability was high (A single pollination rate was 40%).The efficient pollination distance was 60m; The fruit setting rates of apple trees in the west and south were higher than that of apple trees in the east and north in the Osmia pollination orchards. The apples yield and qualities have been improved obviously, comparing with the natural pollination orchards. The Osmia bees liked to neat in the red tube, the nest ratio of bulrush and paper were higher than that of plastic tube, the nest ratio of bulrush was higher than that of paper tube.