Abstract: Enshi mountainous region is one of suitable areas to develop intermediate scent type flue-cured tobacco varieties in China. To find out the elite cultivation modes of intermediate scent tobacco will help increase in the yield and income of local tobacco-producing farmers in Enshi region. In this study, five cultivation modes involving different culture factors, such as transplanting time, nitrogen application level, plant density and remaining leaves, were compared in the growth mode and dry matter accumulation dynamics in the whole growth periods. The results demonstrated that, all five cultivation showed a low-fast-slow pattern of dry matter accumulation in the field growth periods. The highest amount and rate of accumulation appeared from 60 to 75 days after transplantation, leaf had a higher dry matter accumulation rate than stem and root organs, they all conformed to two polynomial regression equations, and also the dry matter accumulation rates was in the order of leaves, stems and root tissues. Path analysis showed that, the dry matter in the leaves was mainly influenced by the maximum leaf area with direct path coefficient 2.152 and stem dry weight with negative value -1.248, the super-high flue-cured tobacco percent was regulated more by starch content with direct coefficient 1.22, reduced sugar with value -0.369 and nicotine contents with value 0.145. However, reduced sugar had a positive value 0.692 by the means of starch and nicotine contents. And thus, cultivation method should be to improve the stem and leaf growths synchronously to keep an appropriate root-shoot ratio, as well as increase the reducing sugar levels, reduce the starch and nicotine contents to obtain the higher super-high tobacco leaf percent. According to the clustering analysis of the yield and chemical component contents in the flue-cured tobacco, the cultivation mode N4 was the best for intermediate scent flue-cured tobacco with intermediate scent type in the Enshi region, potentiating a great application in the cultivation of characteristic tobacco yield and high quality in the future.