Abstract: Trichoderma is a potential biocontrol fungus which was isolated from Griflola frondosa, the morphology and their biological characteristics of Trichoderma were investigated. The results showed that, it could grow on the PDA, MEA, basic and Czapek culture medium, but PDA was the most favorable for the hyphae growth, where the plant can be covered with culture dish in 2 days. From 10℃ to 37℃ it could grow and the optimal temperature for the growth was 25℃, while 30℃ was best for the sporulation. It could grow on the media with a wide range of pH value from 2 to 10, and the optimum pH value was 5 to 7. Beef extract was the optimum nitrogen source and glucose was the best carbon source; VB6 was the best vitamin for hyphae growth.