Abstract: Since many studies have highlighted the feasibility of analyzing DNA from all type of ancient remains including bones, hair, teeth and even coprolithes. Therefore aDNA(ancient DNA) is widely investigated in archeaozoology research fields. However the study of aDNA is challenging due to the contamination by exogenous current DNA. In this paper, we tried to extract the ancient DNA from the ancient meat of Nan’ao Ⅰ shipwreck, and amplify the aDNA by Universal Primers of 18SrDNA and mtDNA 16S rRNA (animal tissue). The designated primers successfully amplified the aDNA .But the results were not reasonable . Through the analysis of the effects of experimental factors, to provide reference for the further research.