Abstract: In order to clone oxalate oxidase gene of soybean and identify its function, the degenerate primer was designed on based on homology oxalate oxidase genes of dicotyledonous plant from GenBank database. Real-time quantitative PCR was used to analyze the gene expression. The cloned gene fragment size was 504 bp without intron and encoded 168 amino acids. Homology comparison found it was high homology with germin protein of soybean and oxalate oxidase of medicago and arachis hypogaca. The gene expression induced by oxalate showed that the expression was relative with oxalate induction. There was significant difference in expression amount and expression time between resistant and susceptive germplasm. To infer that the gene fragment was a germin gene fragment with oxalate oxidase activity.