Abstract: To establish a molecular marker detection system for genetic population construction, a four-step detection system was constructed using SSR molecular marker technology, including DNA extraction, primer polymorphism screening, PCR amplification of parents and offspring, and electrophoresis detection of amplified products. In the experiment, only one pair of primers HvWaxy4 located on barley 7H chromosome was used to detect 7 F1 progenies, three of them were F1 progenies and four were self-pollinated progenies. The results indicated that SSR marker technology was feasible to verify the F1 hybrid plants in the process of genetic population construction, and the method was suitable to detect the authenticity of F1 plants in the process of self-pollinated crops.