Abstract: A method was established to detect 4 kinds of fruit preservatives including p-methyl hydroxybenzoate, p-ethyl hydroxybenzoate, ethyl naphthol and diphenyl ether by High Performance Liquid Chromatography(HPLC).The samples were extracted by ether and purified by activated carbon column.For the determination,reversed phase C18 column was used and mobile phase was 55%CH3OH-45%H2O(pH≈3).A Diode array detector was used at 208nm. Standard addition recovery ranged from 85.4% to 104.7% with relative standard deviation(RSD) from 1.10% to 4.75%. The method was suitable for the detemination of p-methyl hydroxybenzoate, p-ethyl hydroxybenzoate, ethyl naphthol and diphenyl ether residues in fresh fruits with high sensitivity and selectivity.