Abstract: In order to investigate the determination method and content of iron, manganese, copper and zinc in the kernel of macadamia, the content of four trace elements in the kernel of four macadamia cultivars were determined by flames atomic absorption spectrometry (FAAS), the sample were decomposed by three ways include dry ashing, acid digestion, microwave digestion, the content of each trace element in different macadamia cultivars were compared. The results showed that the content of iron and copper determined by three decomposition ways had no significant differences, but the content of manganese and zinc which determined by microwave digestion were slightly higher than the other two decomposition ways, but the differences were not significant. The average content were Mn>Fe>Zn>Cu in the kernel of macadamia, the content of manganese showed a significant difference among cultivars. The iron content of‘YunMac 51’was the highest and‘YunMac 58’had the highest content of manganese, copper and zinc. All the three decomposition ways could be used for trace element determination of macadamia kernel by FAAS.