Abstract: In order to isolate the ubc7 cDNA (Boubc7) from stigma of ornamental kale (Brassica oleracea var. acephala) S13-b homozygotes and obtain the recombinant BoUbc7 fusion protein from E. coli cells. The total RNA was extracted from stigma of ornamental kale using CTAB method. The ubc7 gene was amplified by RT-PCR. The coding region of Boubc7 was inserted into the pET14b and E.coli BL21 (DE3) pLysS cell was transformed pET-14b-BoUbc7. The recombinant BoUbc7 fusion protein was induced by IPTG and purification using Ni2+-NTA resin. Results showed that, the Boubc7 cDNA contained an opening reading frame of 501 bp, encoding a 166 predicted amino acids residue with the predicted molecular mass of 18.7 kDa and the calculated pI of 5.35. SDS-PAGE results showed that the recombinant BoUbc7 fusion protein about 23 kDa was induced by IPTG and purification by affinity chromatography using Ni2+-NTA resin. The Boubc7 gene and recombinant BoUbc7 fusion protein has been obtained, which laid a solid foundation for investigate the biological function of BoUbc7.