Abstract: In order to ascertain genetic background of the high-quality glutinous rice resources, analyze the genetic diversity from the molecular level, and provide a theoretical basis of germplasm identification and innovation use of the high-quality glutinous rice, 14 glutinous rice varieties, including Taihuno and Suyuno from Taihu Lake areas and some other glutinous rice varieties were analyzed by using 17 simple sequence repeats (SSR) primer pairs selected from 35 SSR of National Standard primer pairs that disperse on 12 chromosomes in rice. A total of 63 polymorphic bands were detected among 14 lines. Cluster analysis by using 17 simple sequence repeats (SSR) primer pairs showed that genetics similarities among the 14 lines were above 0.619. The result of cluster reflected basically the genetic relatives based on the pedigree analysis. The DNA fingerprints were constructed by the 6 core SSR primers and the ID card system of these glutinous rice varieties of SSR marker was constructed primarily.