Abstract: The experiment used 3 Lactarius deliciosus collected from GuiZhou and one from Southwestern University as material, PDA can be used to cultivate activation of fungi, Mading Shi can be used to choose the best carbon, nitrogen and pH. By observing the density, color and shape of mycelium, as well as tried dried weighing method to determine the optimal conditions, by comparing the various groups permented strains to determine the best fungi group. We put mycelium on different cultivars prescription, to determine the best cultivars prescription. Experiments showed that: Ld1 is the optimal strain fungi, it can produce large hyphae, PDA can preserve fungi better. The optimal carbon source is glucose, the optimal nitrogen sources is KNO3 and optimal PH is 6. Also with pine sawdust, wheat bran, cottonseed hulls made according to a certain proportion of cultivated species can provide best growth of Lactarius deliciosus .