Abstract: A biomass energy loop utilization project (livestock→ livestock dung→ biogas digester→digestion liquid/ digester residues→ orchard soil) was conducted for five years in typical temperate agro-village of Jixian County in Linfen City, Shanxi Province. The aim of this study was to evaluate the impact of using bio-digestion waste derivatives five years on the physicochemical soil fertility and fruit quality in orchard-soil ecosystem field trials compared with using traditional synthetic fertilization. The results showed that application of bio-digestion waste derivatives induced statistically insignificant enhance apple quality with increased firmness and disaccharide content. The apple firmness and soluble sugar under biogas residues application treatment were increased by 6.3% and 4.7% than that treated with traditional synthetic fertilization. Moreover, the use of these products might stimulate soil fertility and nutrient mobilization from the soil, thereby increasing the use efficiency of soil minerals. The content of total phosphorus, available phosphorus, nitrate nitrogen, available Fe, Cu under biogas residues application treatment were increased by 59.7%, 97.1%, 67.1%, 24.2%, 45.5% than that treated with traditional synthetic fertilization(P<0.05). There were no significant different on dehydrogenase, cellulase and sucrase activities between two type fertilizer application treatments. But the use of biogas fertilizers resulted in the significant decreased by 44.7% and 32.9% in phosphatase and β-glucosidase activities (P<0.05) than that treated with traditional synthetic fertilization. It was clear that the use of biogas residues products as nutrient supply in agriculture should be stimulated to realize apple quality and soil nutrient development of agriculture.