Abstract: The anatomy structures of leaves of 10 strains of wild Camellia sinensis from Guangxi were studied via paraffin-sectioning and optical microtechnique. The study based on the anatomy could help us to explore the ability of anti-drought, anti-cold, and anti-pest, which provided the scientific foundation to the tropical tea resource identifying and new varieties cultivating. The results showed that the thickness of several structures ,including blade, vein, epidermis cells, cuticula, fence form and sponge form significantly varied according to strain. The thickness of the upper epidermic cells ranged from 17.01μm to 29.34μm, and the upper epidermic cuticula ranged from 2.10μm to 5.76 μm. The ratio of thickness of fence form to sponge form ranged from 0.22 to 0.85, while the ratio of vein to blade ranged from 2.46 to 4.66. Further analysis indicated that JB2 and JB5 exhibited strongest ability in anti-cold, drought and pest, followed by JB1, JB3, JL1, respectively, which were considered as the top 5 strain of wild Camellia sinensis with stronger resistance.