Abstract: In order to study the resistance of Escherichia coli isolated from broilers in Fujian. Sterilely resorting to visceral organs, such as livers, spleens, lungs of illness, chicklings that were suspected colibacillosis of each large chicken places in Fujian, by the means of combing with epidemiology, clinical, pathology, biochemistry and PCR method, we could separate and identificate to diagnose whether it was colibacillosis or not. By the means of antimicrobial susceptibility test on 12 bacteria those were separated from part chicken places, we could screen some sensitive drugs and find more effective proprietary to provide theoretical basis and practical parameters of treatment and prevention.