Abstract: In order to choose the index of cold resistance of wheat, the comparison of the dead seedling tillering rate, percentage of withered leaves, leaf number, leaf length and width, plants erect, SPAD value, electrical conductivity was discussed among 12 wheat varieties in the field. The result showed that the dead seedling tillering rate was the reliable identification method, a signification correlation in percentage of withered leaves, plants erect, electrical conductivity with it was observed, the correlation coefficients reached 0.864, 0.609 and 0.578 respectively. Wheat variety with good cold resistance has lower percentage of withered leaves, electrical conductivity and higher creeping, which could be the index of cold resistance identification, although wheat variety with less leaf and higher SPAD value displayed the characteristic of good cold resistance wheat variety, little signification correlation existed, and uncertain relationship of cold resistance emerged in leaf length and width. Therefore, the three indexes as the direct target identification of winter wheat need further study confirmed.