Abstract: This study aimed to explore the influence of Rhodobacter Sphaeroides biofertilizer on degradation of PAHs in soil, accumulation of PAHs in the wheat, and human health-risks of exposing to PAHs. The field experiments were performed by spraying the biofertilizer on leaf surface and irrigating the biofertilizer in root of wheat, with the conventional field management. The results indicated that: the concentration of total PAHs (∑PAHs), compared with the initial soil, had decreased by 21.1% and 27.1%; the equivalent concentrations of benzene [a] pyrene (∑PAHsBaPeq) of∑PAHs had decreased by 69.5% and 66.2% in experiment fields with spraying and irrigating of the biofertilizer, respectively. Compared with the control sample, the concentration of∑PAHs decreased by 45.3% and 49.8%, and the∑PAHsBaPeq decreased by 61.1%, 62.1% in wheat grains. The total lifetime cancer risk, compared with the control, had decreased by 6.5%, 6.7%, and the total loss of life expectancy had decreased by 65.5% and 67.5% in treatment fields, respectively. Thus, the biofertilizer can improve to degradation of PAHs in soil and decrease accumulation of PAHs in wheat grains, consequently, reduce the risk of exposure of people to PAHs and increase the agricultural safety of the PAHs contaminated soil.