Abstract: The study was conducted to set up the cutting propagation technology of Macropanax rosthornii and to provide technical supports for the large-scale production of its nursery seedlings and landscape application. The plant Macropanax rosthornii was used to test the effects of different cuttings, substrates, types and concentrations of growth regulators on its cutting rooting by a conventional cutting experiment. Different cuttings significantly affected their rooting capacity, and 3-year-old hard-wood cuttings had a notably better rooting effect than 1- year- old soft- wood cuttings. Significant difference was found in the rooting effect of substrates, in which yellow subsoil and coconut chaff were better than river sand and chaff but perlite had the poorest rooting effect. The types and concentrations of growth regulators were also important factors that affected cutting rooting. Treated with IBA at a concentration of 2000 mg/L, the cuttings had a rooting rate of 98.8% , root number of 93.75 on an average, root length of 4.23 cm on an average and root effect index of 409.18. And compared with the control, the root number and root effect index were increased by 3.54 times and 4.35 times respectively. The mixture of different growth regulators made against cutting rooting of Macropanax rosthornii. The cutting substrate of yellow subsoil and 3- year- old hard- wood cuttings shortly dipped with 2000 mg/L IBA were appropriate to the cutting rooting of Macropanax rosthornii. The mixture of different growth regulators were not proper to the cutting rooting of Macropanax rosthornii.