Diversity of Endophytic Fungi in Leaves of Different Variety Grapes

doi:10.11924/j.issn.1000-6850.2013-2717

Abstract

Abstract: The research is aimed to study the diversity of endophytic fungi community from different varieties of grape leaves. Cultivable folia endophytic fungi from different varieties of grape leaves were isolated and identified by morphology together with its ITS sequence analysis. In total 1067 strains of cultivable endophytic fungi were isolated from 1215 pieces of grape leaf tissues, and all these fungi strains were identified into 90 species, 28 genus. Colletotrichum and Alternaria were dominant genera with the dominance of 39.08% and 25.21%, respectively. The separation rates of folia endophytic fungi within different varieties of grape varied from 0.56 to 1.13. And the diversity indexes of folia endophytic fungi were varied from 1.32 to 2.39. Concerning the mature degree of grape leaves, mature leaves had the highest level in both isolation rates and diversity indexes than that of the younger leaves and leaves from diseased plants. Isolation rates and diversity indexes were seen significantly different of between one variety to another. The similarities of diversity and structure of folia endophytic fungi between grape varieties were mostly more than 50%. These results implied the existing of host specificity but relatively low diversities of folia endophytic fungi in grapevines.

References

[1] Petrini O. Fungal endophytes of tree leaves. In: Andrews J H, Hirano S S. Microbial Ecology of Leaves[M]. New York: SpringerVerlag,1991:179-197.
[2] 郭良栋.内生真菌研究进展[J].菌物系统,2001,20(1):148-152.
[3] Clay K. Effects of fungal endophytes on the seed and seeding biology of Lolium perenne and Festuca arundinacea[J].Oecologia, 1987,73:358-362.
[4] De Battista J P, Bacon C W, Severson R, et al. Indole acetic acid production by the fungal endophyte of tall fescue [J]. Agronomy Journal,1990,82:878-880.
[5] Kuldaua G, Bacon B C. Clavicipit aceous endophytes: Their ability to enhance resistance of grasses to multiple stresses[J]. Biological Control,2008,46(1):57.
[6] Siegel M R. Acremonium endophytes: our current state of knowledge and future direction for search[J]. Agric Ecosystems Environ,1993,44:301-321.
[7] Marks S, Clay K. Physiological responses of Festuca arundinacea to fungal endophyte infection[J]. New Phytol,1996,133:727-733.
[8] Chen C E, Bauske M, Musson G, et al. Biological control of Fusarium wilt on cotton by use of endophytic bacteria[J]. Biological Control,1995,5:83-91.
[9] Bonnie H Ownley, Mary R Griffin. Beauveria bassiana: Endophyt iccolonization and plant disease control[J].Journal of Inwertebrate Pathology,2008,98(3):267.
[10] Stierle A, Strobel G., Stierle D. Taxonl and taxane production by Ta xamyces andreanae[J]. Science,1993,260:214-216.
[11] Strobel G A, Miller R V, Martinez-Miller C, et al. Cryptocandin, a potent and timycotic from the endophytic fungus Cryptosporiopsis of quercina[J]. Microbiology,1999,145:1919-1926.
[12] Zhang B Salituro G, Szalkowski D, et al. Discovery of small molecule insulin mimetic with antidiabetic activity in mice[J]. Science,1999,284:974-981.
[13] 王文辉,王志华,张志云.世界葡萄发展趋势及产销概况[J].北方果树,2001(3):1-2.
[14] 陈树俊.葡萄的营养与保健[J].农产品加工,2008,10:18-19.
[15] 黄晓东,邵迎,高安娜,等.中国欧亚种葡萄研究文献分析[J].农业图书情报学刊,2005,10(17):136-139.
[16] Kummuang N, Diehl S V, Smith B J, et al. Muscadine grape berry rot diseases in mississippi: disease epidemiology and crop reduction [J]. Plant Disease,1996,80(3):244-247.
[17] 魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979:125- 648.
[18] 邵力平,沈瑞祥,张素轩,等.真菌分类学[M].北京:中国林业出版社, 1984:56-341.
[19] Guo L D，Hyde K D，Liew E C Y． Detection and taxonomic placement of endophytic fungi within frond tissues of Livistona chinensis based on rDNA sequences[J]. Molecular Phylogenetics and Evolution,2001,20:1-13.
[20] Pielou E C. Ecological Diversity[M]. New York: John Wiley and Sons Inc,1975:1-165.
[21] Chapela I H. Fungi in healthy stems and branches of American beech and aspen: a comparative study[J]. New Phytologist, 1989, 113:65-75.
[22] Barengo N, Sieber T N, Holdenrieder O. Diversity of endophytic mycobiota in leaves and twigs of pubescent birch (Betula pubescens) [J]. Sydowia,2000,52:305-320.
[23] Shao S C, Wu S H, Chen Y W, et al. Composition of endophytic fungi in Azadirachta indica from Yuanjing County of Yunnan[J]. Biodiversity Science,2008,16(1):63-67.
[24] Naik B S, Shashikala J, Krishnamurthy Y L. Diversity of fungal endophytes in shrubby medicinal plants of Malnad region, Western Ghats, Southern India[J]. Fungal Ecology,2008,1(2/3):89-93.
[25] 王增福,林筱,钱晓鸣,等.酿酒葡萄内生真菌群落及抗茵活性测定[J].厦门大学学报,2010,49(7):564-569.
[26] Taylor J E, Hyde K D, Jones E B G. Endophytic fungi associated with the temperate palm, Trachycarpus fortunei, within and outside its natural geographic range[J]. New Phytologist,1999,142:335-346.
[27] 翟衡,杜金华,管雪强,等.酿酒葡萄栽培及加工技术[M].北京:中国农业出版社,2001:199-238.
[28] 张晓煜,刘玉兰,张磊,等.气象条件对酿酒葡萄若干品质因子的影响[J].中国农业气象,2007,28(3):326-330.
[29] 曾芹,师俊玲,刘延琳.产白藜芦醇葡萄内生菌的分离与鉴定[J].生物工程,2012,33(13):167-170.
[30] 刘芸,殷红,仇农学.一株产白藜芦醇虎杖内生真菌的分离和鉴定[J].菌物学报,2010,29(4):502-507.
[31] 刘小莉,陈晓红,董明盛.一株银杏内生炭角菌的抗氧化活性研究[J].南京农业大学学报,2008,31(1):102-106.
[32] Mercier J, Reeleder R. Interactions between Sclerotinia sclerotiorum and other fungi on the phylloplane of lettuce[J]. Canadian Journal of Botany,1987,65(8):1633-1637.
[33] Di Pietro A, Gut-Rella M, Paehlatko J P, et al. Role of Antibiotics Produced by Chaetomium globosum in Biocontrol of Ptyhium ultimum. a Causal Agent of Damping Off[J]. PhytoPathology,1992, 82:131-135.