Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (13): 142-146.doi: 10.11924/j.issn.1000-6850.2013-2732

Special Issue: 生物技术 园艺

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Genomic DNA Extraction and RAPD Primer Screening of Pomegranate (Punica granatu .L)

  

  • Received:2013-10-18 Revised:2013-11-20 Online:2014-05-05 Published:2014-05-05

Abstract: The pomegranate cultivars from Pomegranate Germplasm Repository at Zaozhuang in Shandong were used as experimental materials. The genomic DNA was abstracted from pomegranate by CTAB method. The primers were screened by RAPD- PCR amplification. The results showed the genomic DNA was high quality that was suited for RAPD analysis. Twenty 10 bp primers with good polymorphism were screened from fifty RAPD primers. Total 180 bands were amplified from 10 cultivars, in which 134 bands were polymorphic. The percentage of polymorphic band was 74.4%.