Abstract: Along with the rapid development of dairy industry in China, bovine tuberculosis was becoming more and more serious threat to dairy industry. Thus, prevention and control of bovine tuberculosis attracted much more attention nowadays. However, lack of the epidemic data and effective vaccine of tuberculosis posed the greatest obstacle to control bovine tuberculosis in China. Therefore, Dairy herds were intracutaneous inoculated the purified tuberculin and purified paratuberculine and detected by interferon gamma release assay to carry out allergy for a survey of bovine tuberculosis and bovine paratuberculosis in Shandong Province. From 2010 to 2012, we randomly detected 1678 cows in different areas of Shandong Province. The results showed that bovine tuberculosis and paratuberculosis positive rates of herds were reduced from 35.42% and 29.34% in 2010 to 25% and 14.93% in 2012, respectively. It indicated that the tuberculosis and paratuberculosis can be controlled and even purified by monitoring dairy herds, treating positive cows innocuously and isolating suspected cows.