Immunohistochemical Localization of γ-aminobutyric acid A receptor (GABAAR) in the tissues of Carassius auratus gibelio

doi:10.11924/j.issn.1000-6850.2013-2787

Abstract

Abstract: To evaluate the function ofγ- aminobutyric acid A receptor (GABA_AR) in different tissues by observing the specific distribution in central nervous system and peripheral tissues of Carassius auratus gibelio. Using GABA_ARα1 subunit (GABA_ARα1) as the detection indicator of GABA_AR, this study was conducted by immunohistochemical method to investigate the localization of GABA_AR in the telencephalon, mesencephalon, cerebellum, medulla oblongata, liver and kidney of C. auratus gibelio. The results showed that, GABA_AR widely existed in the telencephalon, mesencephalon, cerebellum, medulla oblongata, liver and kidney of C. auratus gibelio. Its highest density was recorded in the cerebellum, and followed by the mesencephalon, medulla oblongata, telencephalon, kidney and liver. Among these tissues, the density of GABA_AR was high in the corpus striatum of telencephalon, the optic tectum of mesencephalon, molecular layer and Purkinje cells of cerebellum, the vagus lobe of medulla oblongata, kidney tubules and hepatocytes. The research revealed that GABA_AR was mainly distributed in central nervous system of C. auratus gibelio, and also had a small amount of distribution in peripheral tissues. This study aimed at laying a scientific basis for the distribution and localization of GABA_AR in C. auratus gibelio.

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