Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (14): 33-38.doi: 10.11924/j.issn.1000-6850.2013-2787

Special Issue: 生物技术

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Immunohistochemical Localization of γ-aminobutyric acid A receptor (GABAAR) in the tissues of Carassius auratus gibelio

  

  • Received:2013-10-28 Revised:2014-03-12 Online:2014-05-15 Published:2014-05-15

Abstract: To evaluate the function ofγ- aminobutyric acid A receptor (GABAAR) in different tissues by observing the specific distribution in central nervous system and peripheral tissues of Carassius auratus gibelio. Using GABAARα1 subunit (GABAARα1) as the detection indicator of GABAAR, this study was conducted by immunohistochemical method to investigate the localization of GABAAR in the telencephalon, mesencephalon, cerebellum, medulla oblongata, liver and kidney of C. auratus gibelio. The results showed that, GABAAR widely existed in the telencephalon, mesencephalon, cerebellum, medulla oblongata, liver and kidney of C. auratus gibelio. Its highest density was recorded in the cerebellum, and followed by the mesencephalon, medulla oblongata, telencephalon, kidney and liver. Among these tissues, the density of GABAAR was high in the corpus striatum of telencephalon, the optic tectum of mesencephalon, molecular layer and Purkinje cells of cerebellum, the vagus lobe of medulla oblongata, kidney tubules and hepatocytes. The research revealed that GABAAR was mainly distributed in central nervous system of C. auratus gibelio, and also had a small amount of distribution in peripheral tissues. This study aimed at laying a scientific basis for the distribution and localization of GABAAR in C. auratus gibelio.